[PDF] Detection, breeding and selection of durable resistance to …
The hypothesis is that pathogenic variants of P. melanocephala develop within the sugarcane production region and parallel the development of new commercial sugar cane cultivars, leading to the diversification of commercial cultivars and identification of horizontal resistance mechanisms will reduce losses to Sugarcane brown rust in the Florida ...
اقرأ أكثرRole of ptsP, orfT, and sss Recombinase Genes in Root …
Screening of the genomic library by hybridization and PCR. ... and mobilized from E. coli S17-(λ-pir) into P. fluorescens Q8r1-96 by using a biparental mating technique. Mutant clones were first selected on LB medium supplemented with rifampin, kanamycin, and 5% sucrose. ... Functional analyses have shown that this chain is involved in sugar ...
اقرأ أكثرSugarcane Breeding in Australia | SpringerLink
Sugarcane is an important crop to the Australian economy, and the plant breeding program has contributed significantly to the success of the sugarcane industry. Currently, the Australian sugarcane breeding program is conducted at 6 research stations by Sugar Research Australia in collaboration with Wilmar Sugar. Crossing is mainly conducted in …
اقرأ أكثرComparison of Benefit to Sugarcane Plant Growth and 15N2 …
The ability of the nitrogen-fixing bacterial endophyte Acetobacter diazotrophicus strain PAl5 to enhance the growth of sugarcane SP70-1143 was evaluated in the growth chamber, greenhouse, and field by comparing plants inoculated with wild-type and Nif¯ mutant MAd3A in two independent experiments. The wild-type and Nif¯ mutant strains colonized …
اقرأ أكثرDirect screening of recombinants in gram-positive bacteria using …
A system for direct screening of recombinant clones in Lactococcus lactis, based on secretion of the staphylococcal nuclease (SNase) in the organism, was developed. The nuc gene (encoding SNase) was cloned on both rolling-circle and theta-replicating plasmids. ... in which direct screening of nuc mutant recombinant clones was also achieved. The ...
اقرأ أكثرScreening of Bi-parental and Mutant Clones of Sugarcane …
The results of the application of the standard smut disease scale of sugarcane showed that all the clones from bi-parental crosses and only one clone (KRS/96/002) from gamma irradiated progenies were highly resistant to smut disease of sugarcane with zero incidence of the disease.
اقرأ أكثرIsolation and characterization of mutants of Escherichia coli …
Screening of about 30,000 mutagenized clones of a uvr-B derivative of AB1157 yielded six Umu- strains. The mutants can be classified into three groups by the location of the mutations, umuA, umuB and umuC. Mutations umuA and umuB are, respectively, mapped close to lexA and recA genes and mutations at both loci partially reduce UV mutagenesis ...
اقرأ أكثرMarkers associated with stalk number and suckering in …
Molecular markers linked to both of these traits are sought to assist in the identification of high sugar yield, high stalk number, low-suckering sugarcane clones. In this preliminary mapping study, 108 progeny from a biparental cross involving two elite Australian sugarcane clones were evaluated at two sites for two years for both stalk number ...
اقرأ أكثرBSA and molecular markers screening for salt stress tolerant mutant …
BSA and molecular markers screening for salt stress tolerant mutant of Petunia ... V.Y. et al. Molecular profiling using RAPD technique of salt and drought tolerant regenerants of Sugarcane. Sugar Technology, v.8, p.63-68, 2006. ... -induced mutant clones and one bulked DNA sample of non-mutated clone of Petunia×atkinsiana D. Don. cv. Prism ...
اقرأ أكثرThe art and design of genetic screens: Drosophila melanogaster
The mutant clones now occupy almost all of the eye, because they outcompete the slow-growing non-recombinant cells (which are M/+), whereas the twin-spot clones die.
اقرأ أكثرAgronomy | Free Full-Text | Development and Use of Simple
Sugarcane (Saccharum spp.) is a major global crop, not only required for the production of biofuels such as ethanol, but it also produces 80% of total dietary sugar globally [], and 92% of the sugar consumed in China [].Modern cultivars exhibit complex aneu-polyploid genomes with chromosome numbers ranging from 100 to 130, and are inter-specific hybrids …
اقرأ أكثرScreening of Bi-parental and Mutant Clones of Sugarcane …
Six newly developed clones of sugarcane (Saccharum officinarum L.) clones obtained from gamma irradiated progenies (KRS/96/007, KRS/96/002, KRS/96/001) and from bi-parental crosses (SRS/96/305,...
اقرأ أكثرDetecting, Cloning, and Screening for Suppressors of RNA Silencing …
Maize lethal necrosis disease (MLND) is one of the most important viral diseases of maize. MLND occurs when Maize chlorotic mottle virus (MCMV) co-infects the same plant with one of several potyviruses, including Sugarcane mosaic virus, Wheat streak mosaic virus or Maize dwarf mosaic virus. Originally prevalent in the Midwest and Peru in the 1970s, the disease was called corn …
اقرأ أكثرUse of isogenic human cancer cells for high-throughput screening …
The choice of a single clone of each cell line for drug screening was based on bright and uniform fluorescent protein expression. The E1A immortalized RK3E cell line was obtained from American ...
اقرأ أكثرThe Selection of Sugarcane Families That Display Better Associations ...
In particular, four months after germination, 54 families from crosses between clones of sugarcane were treated either with an inoculant named Triazo, which was composed of a mixture of the Abv5, Abv6 and Abv7 strains of A. brasilense, or with the IC26 strain of A. brasilense. The treated plants were then planted in fields.
اقرأ أكثرSugarcane (Saccharum spp.): Breeding and Genomics
Sugarcane (Saccharum spp.) is cultivated and credited worldwide for its ability to synthesize and store exceptionally high concentration of sucrose.Since prehistoric times sugarcane cultivation has undergone many transformations into present-day noble cane. Initially, selection of desirable clones and interspecific hybrids brought many agronomically-useful …
اقرأ أكثرRESEARCH INTRODUCTION<SUGAR<JAMSTEC
High-throughput screening (HTS) for a gene mutagenesis library is like "looking for a needle in the haystack.". While a four-character idiom with the same meaning in Chinese is "looking for a needle in the ocean.". The scientist …
اقرأ أكثرMutagenesis and Identification of Sugarcane Mutants Using ... - Hindawi
The KK3 sugarcane cultivar was used as a control for the experiment. The mutant sugarcane clones for water-stress tolerance were identified by comparing their leaf damage scores with the leaf damage score of KK3. ... respectively. According to this finding, the MS media containing 15 and 20% of PEG were used for screening mutant calli for water ...
اقرأ أكثرImprovements of the productivity and saccharification efficiency of …
Expression of recombinant D2-BGL in S. cerevisiae and P. pastoris. Saccharomyces cerevisiae is commonly used for the construction of mutant libraries via random mutagenesis because of its ability to reconstitute linearized DNA fragments into plasmids by homologous recombination []. P. pastoris is frequently used in industry for large-scale …
اقرأ أكثرGel microdroplet–based high-throughput screening for
The selection model was further improved by reducing the amount of target strain to 0.01% of the total cells. Sorted droplets were spread on two BMMY screening plates containing 1% (w/v) xylan and incubated at 30°C for 48 h. The Xylan-Congo red clearance plate assay was adopted to qualitatively show that the clones were xylanase-producing strains.
اقرأ أكثرHigh-Throughput Differentiation and Screening of a …
We used a genomic library of mutant murine embryonic stem cells (ESCs) and report the methodology required to simultaneously culture, differentiate, and screen more than 3,200 heterozygous mutant clones to identify host-based genes involved in both sensitivity and resistance to rabies virus infectio …
اقرأ أكثرMutagenesis and Identification of Sugarcane Mutants Using
Over the 50-year period that the two crossing methods were used, the melting pot method proved to be more labour efficient and ultimately contributed more than the biparental crossing procedure toward the development of new commercial cultivars for …
اقرأ أكثرEvaluation of Resistance to Leaf Scald by Quantitative PCR of ...
Plant material and sample collection. Thirty-one sugarcane clones were included to compare quantification of X. albilineans by TaqMan qPCR and rating resistance based on symptom severity. The clones included three known leaf-scald-resistant clones (LCP 85-384, Ho 95-988, and HoCP 96-540) and two susceptible clones (HoCP 85-845 and HoCP 86-849) as …
اقرأ أكثرRapid screening of sugar-nucleotide donor specificities of …
GT reaction schematic. (Top) A simplified reaction where a GT catalyzes the transfer of a carbohydrate moiety from a specific donor substrate to an acceptor molecule.For screening of sugar-nucleotide donors, a GT is incubated with a panel of donor sugars in the absence of an acceptor substrate (Bottom), and the UDP released upon background …
اقرأ أكثرScreening for thermostable mutant of kanamycin nucleotidyltransferase ...
Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus ... Out of greater than 8 X 10(3) transformants, 12 clones that were suspected to harbor the mutant gene encoding the more thermostable enzyme were isolated by shifting from a permissive (55 ...
اقرأ أكثرBlue-White Screening & Protocols for Colony Selection - Sigma-Aldrich
For screening the clones containing recombinant DNA, a chromogenic substrate known as X-gal is added to the agar plate. If β-galactosidase is produced, X-gal is hydrolyzed to form 5-bromo-4-chloro-indoxyl, which spontaneously dimerizes to produce an insoluble blue pigment called 5,5'-dibromo-4,4'-dichloro-indigo.
اقرأ أكثرHistory of Sugarcane Breeding, Germplasm Development and …
Sugarcane is one of the four major crops of Myanmar, and its breeding and related research has begun with the establishment of Pyinmana Central Farm, Naypyitaw, in 1925. The key objectives of sugarcane breeding program in Myanmar are to produce sugarcane varieties with improved cane and sugar yield, good ratooning ability and resistance to locally …
اقرأ أكثرA high-throughput pH indicator assay for screening …
Protein engineering using directed evolution or saturation mutagenesis is a powerful technique for altering biocatalyst specificity, activity, and stability,,, .A critical aspect of this approach is the development of a robust screening system to identify mutants with the desired properties .Whereas numerous assays for glycosyltransferases have been developed, …
اقرأ أكثرFrom the Cover: Development and application of a suite of ...
Interestingly, another irx mutant, irx7 also referred to as fra8, displayed a very similar sugar composition as irx9 and was shown to be involved in biosynthesis of glucuronoxylan . ... Screening of recombinant clones, expression optimization, and purification of enzymes were done as described .
اقرأ أكثرBreeding Methods in Crop Plants | Plant Breeding | Botany
Mutation breeding is common in self-pollinated and asexually propagated species and rare in cross pollinated species. A mutant variety differs from parent variety in one or few characters. A Mutant differs from a segregant in two main ways. Firstly, the frequency of segregants is very high and that of mutant is extremely low (0.1%).
اقرأ أكثرRESEARCH CENTERS - Sugarcane Breeding Institute, Coimbatore, …
A mutant of Co 997 (Co 997M) which was free from natural incidence of red rot was released; 1971-80: ... A database on winter ratoonability of sugarcane clones was published; The Central Varietal Release Committee released Co 98014 (Karan 1) as an early variety. ... Unlike the inter-varietal crosses through biparental mating, distant ...
اقرأ أكثرIdentification of type II and type III pyoverdine receptors …
Triparental mating between pooled clones of the bank, a helper E. coli strain containing plasmid pRK 2013 and mutant 59.20-18B3 was performed in order to complement strain 59.20-18B3. The complemented mutant was selected on CAA plus EDDHA. The cosmid clone 2E7 that complemented the mutant was isolated from the library and the cosmid DNA was ...
اقرأ أكثرScreening for Resistance to Sugarcane Brown Rust With …
Inoculation of sugarcane clones for brown rust resistance evaluation. Plants of 21 sugarcane clones were produced vegetatively from single-node cuttings in the greenhouse and used in three repeated experiments to evaluate the ability of inoculation under controlled conditions to determine and compare brown rust resistance ratings in clones with variable …
اقرأ أكثر4 In vitro mutagenesis and downstream screening …
This program utilized S. officinarum, S. spontaneum, S. barberi, Sugar Tech S. sinense, S. robustum, and ISH derivatives, as parents. Several sugarcane cultivars of …
اقرأ أكثرCharacterization of IDH1 p.R132H Mutant Clones Using Mutation …
Isocitrate dehydrogenase 1 (IDH1) and IDH2 mutations occur in a variety of myeloid neoplasms. Immunohistochemistry (IHC)-based direct visualization of mutant clones of hematopoietic cells can be useful for rapid diagnostic screening and for monitoring treatment response. In …
اقرأ أكثر4 In vitro mutagenesis and downstream screening strategies in sugarcane …
Several of the sugarcane cultivars Co 281, Co 290, Co 221, Co 213, Co 244, Co 312 and Co 313 are hybrids of S. officinarum, S. spontaneum and S. barberi.
اقرأ أكثرHigh-throughput screening methodology for the directed ... - Nature
We confirmed the ability of the F91Y mutant to specifically form an α-2,3 glycosidic linkage by TLC detection of bodipy lactose formation …
اقرأ أكثرFunctional-Based Screening Methods for Detecting ... - SpringerLink
The function-driven analysis of metagenomes or clone mutant libraries is an interesting approach for searching and retrieving enzymes from a medium to high scale. For accessing to the wide functional diversity, one should consider the following concept: the more general screening method used the widest enzyme diversity you can retrieve.
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